What is fluoro-jade c?

Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons.

How does fluoro-Jade C work?

Fluoro-Jade® C stains all degenerating neurons regardless of specific insult or mechanism of cell death. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals.

What does Fjb stain?

Fluoro-Jade B (FJB) is an anionic fluorescein derivative that has been reported to specifically stain degenerating neurons.

What is the history of fluoro-jade staining?

The first fluoro-jade derivative was reported by Larry Schmued in 1997 as an alternative method from traditional methods for labeling degenerating neurons such as silver nitrate staining, H&E stain, or Nissl stain.

What is fluoro-jade B and fluoro-jade C?

The newer dyes, fluoro-jade B and fluoro-jade C, were developed to improve signal to noise ratio, therefore creating superior compounds for visualizing finer neuronal morphology including dendrites, axons and nerve terminals.

How do you dilute fluoro-jade?

Fluoro-Jade is highly soluble in water and is therefore first dissolved in distilled water. In order to be specific for degenerating neurons, fluoro-jade must be used in an acidic environment, therefore fluoro-jade is further diluted in glacial acetic acid.

How do fluoro-jade dyes label degenerating neurons?

However, the mechanism by which fluoro-jade labels degenerating neurons is unknown thus creating some controversy to the actual physiological condition of the labeled cells. Currently, there are three fluoro-jade dyes (Fluoro-Jade, Fluoro-Jade B, and Fluoro-Jade C ), all of which are anionic derivatives of fluorescein and highly acidic.